Biologist

(ID: 2026-1840) Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH). Axle is seeking a Part-Time B iologist to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute on Aging (NIA) located in Baltimore, MD. Benefits We Offer: 100% Medical, Dental & Vision Coverage for Employees Paid Time Off and Paid Holidays 401K match up to 5% Educational Benefits for Career Growth Employee Referral Bonus Flexible Spending Accounts: Healthcare (FSA) Parking Reimbursement Account (PRK) Dependent Care Assistant Program (DCAP) Transportation Reimbursement Account (TRN) Position Information: Provide support services to satisfy the overall operational objectives of the National Institute on Aging. The primary objective is to provide services and deliverables through performance of support services. Additional Qualifications: Certifications & Licenses Bachelor’s degree in Biology, Life Sciences or a related discipline Field of Study Biology Software MS office suite Skills Ability to communicate both orally and in writing. Deliverables: Work products and documents related to cultivating, maintaining, expanding and freezing ES and iPS cell lines from mouse and human; prepare ES and iPS cells for Nlinked glycoprotein labeling and analysis and for flow analyses; characterize new iPS cell lines; investigate state of pluripotency of ES and new iPS cell lines; characterize nuclear proteins and cell surface proteins by western blot, immunoprecipitation, immunochemistry, and PCR. - Ad-Hoc Work products and documents related to identifying/recommending resolution of discrepancies; recommend/determine the appropriate data to use, record or report. - Ad-Hoc Work products and documents related to creating/maintaining an accurate research tracking system and database for all research activities; keep accurate records of daily experiments and results; prepare reports, spreadsheets and slides for presentation and/ or publication; write and edit scientific papers; present ongoing findings at various meetings. - Ad-Hoc Work products and documents related to performing routine maintenance on laboratory instruments and equipment; ensure supplies are properly stocked; obtain quotes and prepare purchase requests. - Ad-Hoc Clean Equipment - Ad-Hoc Inspect Equipment - Ad-Hoc Meet with lab members to present updates - Weekly Statement of Work Details: Program and use computers to store, process, and analyze data - 1 Interpret research or operational data - 2 Record research or operational data - 3 Collect and analyze biological data about relationships among and between organisms and their environment - 4 Monitor and observe experiments, recording production and test data for evaluation by research personnel - 5 Provides technical experience needed to assist in studying the basic principles of plant and animal life Study basic principles of plant and animal life, such as origin, relationship, development, anatomy, and function Identify, classify, and study structure, behavior, ecology, physiology, nutrition, culture, and distribution of plant and animal species Assists with performing basic laboratory experiments and procedure Analyze experimental data and interpret results to write reports and summaries of findings Cultivate, maintain, expand and freeze ES and iPS cell lines from mouse and human Prepare ES and iPS cells for N-linked glycoprotein labeling and analysis by Mass Spectrometry Prepare ES and iPS cells for flow analyses, including the labeling of the cells with appropriate antibodies Generate primary cultures of cells for the generation of new iPS cell lines Characterize new iPS cell lines using appropriate techniques (morphology, staining, growth traits) Prepare primary cell cultures suitable for iPS cell formation using standard nucleofection based technique) Perform primary cell cultures of mitotically inactivated feeder layers Design and execute flow cytometry (FACS) preps, proliferation assay, and cell surface receptor analysis Investigate state of pluripotency of ES and new iPS cell lines Characterize nuclear proteins and cell surface proteins by western blot, immunoprecipitation, immunochemistry, and PCR Design primers as needed for cell characterization Apply a wide range of conventional, established approaches, methods, techniques and solutions to new situations; identify/recommend resolution of discrepancies; recommend/ determine what data to use, record or report Prepares, organizes and maintains various lab samples, supplies, and equipment Maintain laboratory equipment Analyze laboratory findings Maintain an accurate research tracking system and database for research activities; keep accurate records of daily experiments and results including research documentation Prepare data, results, reports as well as Excel spreadsheets and PowerPoint slides, for oral presentation and/or publication in journals; write and edit scientific papers Attend monthly laboratory meetings; participate in lab/unit/section meetings by presenting ongoing research and findings; present paper/abstract/poster at scientific meeting(s)/conference(s) Operate and perform routine maintenance on laboratory instruments and equipment; ensure scientific supplies are kept stocked; obtain quotes and prepare purchase requests for supplies, instruments and equipment, and submit to primary investigator and/or lab manager for approval Learns new laboratory techniques and procedures Cultivate, maintain, expand and freeze ES and iPS cell lines from mouse and human Prepare ES and iPS cells for N-linked glycoprotein labeling and analysis by Mass Spectrometry Prepare ES and iPS cells for flow analyses, including the labeling of the cells with appropriate antibodies Generate primary cultures of cells for the generation of new iPS cell lines Characterize new iPS cell lines using appropriate techniques (morphology, staining, growth traits) Prepare primary cell cultures suitable for iPS cell formation using standard nucleofection based techniques Perform primary cell cultures of mitotically inactivated feeder layers Design and execute flow cytometry (FACS) preps, proliferation assay, and cell surface receptor analysis Investigate state of pluripotency of ES and new iPS cell lines Characterize nuclear proteins and cell surface proteins by western blot, immunoprecipitation, immunochemistry, and PCR Design primers as needed for cell characterization Apply a wide range of conventional, established approaches, methods, techniques and solutions to new situations; identify/recommend resolution of discrepancies; recommend/ determine what data to use, record or report Maintain an accurate research tracking system and database for research activities; keep accurate records of daily experiments and results including research documentation Prepare data, results, reports as well as Excel spreadsheets and PowerPoint slides, for oral presentation and/or publication in journals; write and edit scientific papers Attend monthly laboratory meetings; participate in lab/unit/section meetings by presenting ongoing research and findings; present paper/abstract/poster at scientific meeting(s)/conference(s) Operate and perform routine maintenance on laboratory instruments and equipment; ensure scientific supplies are kept stocked; obtain quotes and prepare purchase requests for supplies, instruments and equipment, and submit to primary investigator and/or lab manager for approval Disclaimer: The above description is meant to illustrate the general nature of work and level of effort being performed by individuals assigned to this position or job description. This is not restricted as a complete list of all skills, responsibilities, duties, and/or assignments required. Individuals may be required to perform duties outside of their position, job description or responsibilities as needed. The diversity of Axle’s employees is a tremendous asset. We are firmly committed to providing equal opportunity in all aspects of employment and will not tolerate any illegal discrimination or harassment based on age, race, gender, religion, national origin, disability, marital status, covered veteran status, sexual orientation, status with respect to public assistance, and other characteristics protected under state, federal, or local law and to deter those who aid, abet, or induce discrimination or coerce others to discriminate. Accessibility: If you need an accommodation as part of the employment process please contact: [email protected] This role has a market-competitive salary with an anticipated base compensation range listed below. Actual salaries will vary depending on a candidate’s experience, qualifications, skills, and location. Salary Range $70 — $74 USD

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